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Addgene: Homepage
Addgene now distributes ready-to-use recombinant antibodies. Browse our growing collection and learn more about our antibody production process, quality control, and open access to sequences. Addgene's CRISPR collection includes plasmids and lentiviral preps for gene disruption, RNA base editing, pooled library screening, and more.
Addgene.orgDA: 15 PA: 15 MOZ Rank: 16
Addgene: Molecular Biology Reference
Many plasmids are designed to include an antibiotic resistance gene, which when expressed, allows only plasmid-containing bacteria to grow in or on media containing that antibiotic. These antibiotic resistance genes not only give the scientist with an easy way to detect plasmid-containing bacteria, but also provide those bacteria with a pressure to maintain and replicate …
Addgene.orgDA: 15 PA: 19 MOZ Rank: 35
Addgene: Till Strowig Lab Materials
The Till Strowig Lab has deposited materials at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research. Learn more about research in the Till Strowig Lab. Addgene Alerts Receive email alerts when new plasmids from this lab become available. ...
Addgene.orgDA: 15 PA: 14 MOZ Rank: 31
Addgene: Protocol - Over-Agar Antibiotic Plating
Plate 50 µL of transformed E. coli/rescue media suspension onto the agar and gently spread over the surface until the liquid is mostly absorbed. The spreading of cells can be done in the same way as the antibiotic, using either a bent micropipette tip or other cell spreading device that fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2. Observe plates for …
Addgene.orgDA: 15 PA: 40 MOZ Rank: 58
Save Time and Money by Making Your Own Competent …
The competent cells are then aliquoted and stored at -80 o C until we are ready to perform transformations. A typical 200mL bacterial culture produces 20mL of competent cells, enough for 800 transformations when using 25uL competent cells per reaction. We grow our cells in LB rather than the ZymoBroth TM, so it costs us less than 5 cents per ...
Blog.addgene.orgDA: 16 PA: 50 MOZ Rank: 75
media.addgene.org
Media.addgene.org ... SnapGene ß ...
Media.addgene.orgDA: 17 PA: 50 MOZ Rank: 45
media.addgene.org
{ "ORFs": [ { "fullRangeBegin": 198, "fullRangeEnd": 731, "hitsStopCodon": true, "id": 0, "lacksStartCodon": false, "molecularWeight": 20036.050000000003 ...
Media.addgene.orgDA: 17 PA: 50 MOZ Rank: 44
media.addgene.org
Media.addgene.org ... o ( ( (
Media.addgene.orgDA: 17 PA: 50 MOZ Rank: 82
Home > Newsletters > Techniques Newsletter Techniques
Be to take the very popular retroviral vector pBabe-puro (Addgene plasmid #1764) which has a relatively limited MCS (BamHI - SnaBI - EcoRI - SalI) and expand that with the addition of NdeI, PacI, AscI, and MfeI to drastically improve the MCS - and best of all - for maximal compatibility they all work in NEB buffer 4. Briefly, we will design overlapping oligos that once annealed can …
Media.addgene.orgDA: 17 PA: 42 MOZ Rank: 67
LentiCRISPRv2 and lentiGuide-Puro: lentiviral CRISPR/Cas9 and
AddGene 19319). Target design notes and online resources: For application of Cas9 for site-specific genome editing in eukaryotic cells and organisms, we have computationally identified suitable target sites for the S. pyogenes Cas9 and calculated most likely off-targets within the genome. Please visit ...
Media.addgene.orgDA: 17 PA: 50 MOZ Rank: 83
Summer Fun at Addgene!
We’re very excited to be launching the official Addgene TikTok at the end of this month! We’ll be sharing our educational resources and tips and tricks for those new to the lab, along with some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing….
Blog.addgene.orgDA: 16 PA: 22 MOZ Rank: 48
Why Add Sucrose? Improved Yields for Adeno-associated Virus
In our study we observed that low doses of sucrose improve AAV2 yields approximately 1.8-fold without affecting cell viability. This effect appears to be serotype specific as sucrose treatment also improved yields of the AAV2 derivative, rAAV2-retro, but had no effect on AAV5 yields. We further show that this effect is not specific to sucrose ...
Blog.addgene.orgDA: 16 PA: 50 MOZ Rank: 82
Addgene Newsletter Opt-In
By selecting the box below, I am agreeing to allow Addgene, via its newsletter service provider, Hubspot, to send Addgene's bi-monthly newsletter at the email address I provided above (until I opt out of receiving Addgene's newsletter or Addgene decides to no longer publish newsletters). Please note that the email frequency is subject to change ...
Info.addgene.orgDA: 16 PA: 26 MOZ Rank: 54
Early Career Researcher Toolbox: Social Media for Scientists
3. "Social Media as a Scientist: A Very Quick Guide" from Nature Jobs Blog. While Twitter is a popular platform for scientists, it’s not the only tool in a scientist’s social media toolbox. Besides advice for using Twitter, this post provides suggestions for using LinkedIn and Facebook. For each of the three social media platforms, there ...
Blog.addgene.orgDA: 16 PA: 50 MOZ Rank: 89
Sign up to receive Addgene's Science Career Guide
By selecting the box below, I am agreeing to allow Addgene, via its email service provider, Hubspot, to send the Science Career Guide, updates to the guide, and Addgene's bi-monthly newsletter (if the applicable box is selected above) at the email address I provided above (or until I opt of receiving information about the Science Career Guide or Addgene's newsletter).
Info.addgene.orgDA: 16 PA: 49 MOZ Rank: 79
Download Addgene's eBook: CRISPR 101
By selecting the box below, I am agreeing to allow Addgene, via its email service provider, Hubspot, to send the CRISPR 101 eBook, updates to the eBook, and Addgene's bi-monthly newsletter (if the applicable box is selected above) at the email address I provided above (or until I opt of receiving information about the CRISPR 101 eBook or ...
Info.addgene.orgDA: 16 PA: 47 MOZ Rank: 78
Download Addgene's Newly Revised eBook: Plasmids 101
Fill out this form to download the eBook today. Check this box if you'd also like to receive Addgene's newsletter! By selecting the box below, I am agreeing to allow Addgene, via its email service provider, Hubspot, to send the Plasmids 101 eBook, updates to the eBook, and Addgene's bi-monthly newsletter (if the applicable box is selected above ...
Info.addgene.orgDA: 16 PA: 49 MOZ Rank: 81
Download Addgene's Viral Vectors 101 eBook
Download Addgene's free Viral Vectors 101 eBook to learn all about how you can use AAV, lentivirus, retrovirus, and adenovirus in your experiments! Download Addgene's Viral Vectors 101 eBook We've collected the articles from our viral vectors blog series, gotten input from experts, developed new content in-house, and formatted everything into this comprehensive resource.
Info.addgene.orgDA: 16 PA: 50 MOZ Rank: 85
Plasmids 101: Optimizing Plasmid Yields - Addgene
This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your stab from Addgene, streak for single colonies, sub-culture, and prep with a DNA prep kit or your lab's favorite in-house protocol.DNA yields for this procedure are typically in excess of 100 ng/ul, more than enough DNA to verify your plasmid via sequencing or restriction digest.
Blog.addgene.orgDA: 16 PA: 39 MOZ Rank: 73
Addgene - Our recent AAV webinar was fantastic. In fact,.
Our recent AAV webinar was fantastic. In fact, we had so many questions that we couldn't get to them all in the allotted period. But here at Addgene, and at the CLOVER center, we hate seeing...
Facebook.comDA: 16 PA: 32 MOZ Rank: 67
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